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pet his6 gst tev lic cloning vector  (Addgene inc)


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    Structured Review

    Addgene inc pet his6 gst tev lic cloning vector
    Pet His6 Gst Tev Lic Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+his6+gst+tev+lic+cloning+vector/pET+His6+GST+TEV+LIC+cloning+vector+(1G)+(Plasmid+%2329655)/pmc13008262-280-9-17
    Average 93 stars, based on 21 article reviews
    pet his6 gst tev lic cloning vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Membrane composition-dependent patterning of Rho and F-actin in an artificial cell cortex
    Article Snippet: GFP-C1-PLCδ-PH was a gift from Tobias Meyer (Addgene plasmid #21179; Stauffer et al. , 1998 ). .. The PH domain from GFP-C1-PLCδ-PH was subcloned into the pET His6 GST TEV LIC cloning vector (1G; Addgene plasmid #29655, a gift from Scott Gradia) to generate 12XHis-GST-GFP-PH. .. 12XHis-GST-GFP-PH was recombinantly expressed in E. coli Star cells (Invitrogen C601003).

    Article Title: MRTF specifies a muscle-like contractile module in Porifera
    Article Snippet: Polyclonal antibodies against stMyHC and MRTF were produced in rabbits (Syd Labs), and polyclonal antibodies against TAGNL2 and TAGNL3 were produced in chickens (Pacific Immunology). .. The coding region of each antigen (Supplemental Sequences) was cloned into the pET His6 GST TEV LIC cloning vector (Addgene, plasmid #29655) from synthesized gBlocks (Integrated DNA technologies) (stMyHC) or from the E. muelleri cDNA library. .. Recombinant proteins were expressed in Escherichia coli (Rosetta strain DE3; EMD Millipore) and purified with Pierce Glutathione resin (ThermoFisher Scientific #16101) following the manufacturer’s protocol.

    Article Title: Reconstitution of autophagic-like membrane tethering reveals that Atg11 can bind and cluster vesicles on cargo mimetics.
    Article Snippet: Macroautophagy (hereafter, autophagy) is essential for the degradation of mitochondria from yeast to humans.. Mitochondrial autophagy in yeast is initiated when the selective autophagy scaffolding protein Atg11 is recruited to mitochondria through its interaction with the selective autophagy receptor Atg32.. This also results in the recruitment of small 30-nm vesicles that fuse to generate the initial phagophore membrane.

    Plasmid Preparation:

    Article Title: Membrane composition-dependent patterning of Rho and F-actin in an artificial cell cortex
    Article Snippet: GFP-C1-PLCδ-PH was a gift from Tobias Meyer (Addgene plasmid #21179; Stauffer et al. , 1998 ). .. The PH domain from GFP-C1-PLCδ-PH was subcloned into the pET His6 GST TEV LIC cloning vector (1G; Addgene plasmid #29655, a gift from Scott Gradia) to generate 12XHis-GST-GFP-PH. .. 12XHis-GST-GFP-PH was recombinantly expressed in E. coli Star cells (Invitrogen C601003).

    Article Title: MRTF specifies a muscle-like contractile module in Porifera
    Article Snippet: Polyclonal antibodies against stMyHC and MRTF were produced in rabbits (Syd Labs), and polyclonal antibodies against TAGNL2 and TAGNL3 were produced in chickens (Pacific Immunology). .. The coding region of each antigen (Supplemental Sequences) was cloned into the pET His6 GST TEV LIC cloning vector (Addgene, plasmid #29655) from synthesized gBlocks (Integrated DNA technologies) (stMyHC) or from the E. muelleri cDNA library. .. Recombinant proteins were expressed in Escherichia coli (Rosetta strain DE3; EMD Millipore) and purified with Pierce Glutathione resin (ThermoFisher Scientific #16101) following the manufacturer’s protocol.

    Article Title: Reconstitution of autophagic-like membrane tethering reveals that Atg11 can bind and cluster vesicles on cargo mimetics.
    Article Snippet: Macroautophagy (hereafter, autophagy) is essential for the degradation of mitochondria from yeast to humans.. Mitochondrial autophagy in yeast is initiated when the selective autophagy scaffolding protein Atg11 is recruited to mitochondria through its interaction with the selective autophagy receptor Atg32.. This also results in the recruitment of small 30-nm vesicles that fuse to generate the initial phagophore membrane.

    Clone Assay:

    Article Title: MRTF specifies a muscle-like contractile module in Porifera
    Article Snippet: Polyclonal antibodies against stMyHC and MRTF were produced in rabbits (Syd Labs), and polyclonal antibodies against TAGNL2 and TAGNL3 were produced in chickens (Pacific Immunology). .. The coding region of each antigen (Supplemental Sequences) was cloned into the pET His6 GST TEV LIC cloning vector (Addgene, plasmid #29655) from synthesized gBlocks (Integrated DNA technologies) (stMyHC) or from the E. muelleri cDNA library. .. Recombinant proteins were expressed in Escherichia coli (Rosetta strain DE3; EMD Millipore) and purified with Pierce Glutathione resin (ThermoFisher Scientific #16101) following the manufacturer’s protocol.

    Article Title: Reconstitution of autophagic-like membrane tethering reveals that Atg11 can bind and cluster vesicles on cargo mimetics.
    Article Snippet: Macroautophagy (hereafter, autophagy) is essential for the degradation of mitochondria from yeast to humans.. Mitochondrial autophagy in yeast is initiated when the selective autophagy scaffolding protein Atg11 is recruited to mitochondria through its interaction with the selective autophagy receptor Atg32.. This also results in the recruitment of small 30-nm vesicles that fuse to generate the initial phagophore membrane.

    Synthesized:

    Article Title: MRTF specifies a muscle-like contractile module in Porifera
    Article Snippet: Polyclonal antibodies against stMyHC and MRTF were produced in rabbits (Syd Labs), and polyclonal antibodies against TAGNL2 and TAGNL3 were produced in chickens (Pacific Immunology). .. The coding region of each antigen (Supplemental Sequences) was cloned into the pET His6 GST TEV LIC cloning vector (Addgene, plasmid #29655) from synthesized gBlocks (Integrated DNA technologies) (stMyHC) or from the E. muelleri cDNA library. .. Recombinant proteins were expressed in Escherichia coli (Rosetta strain DE3; EMD Millipore) and purified with Pierce Glutathione resin (ThermoFisher Scientific #16101) following the manufacturer’s protocol.

    cDNA Library Assay:

    Article Title: MRTF specifies a muscle-like contractile module in Porifera
    Article Snippet: Polyclonal antibodies against stMyHC and MRTF were produced in rabbits (Syd Labs), and polyclonal antibodies against TAGNL2 and TAGNL3 were produced in chickens (Pacific Immunology). .. The coding region of each antigen (Supplemental Sequences) was cloned into the pET His6 GST TEV LIC cloning vector (Addgene, plasmid #29655) from synthesized gBlocks (Integrated DNA technologies) (stMyHC) or from the E. muelleri cDNA library. .. Recombinant proteins were expressed in Escherichia coli (Rosetta strain DE3; EMD Millipore) and purified with Pierce Glutathione resin (ThermoFisher Scientific #16101) following the manufacturer’s protocol.



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